We made a new glutamate indicator with improved sensitivity, kinetics & localization for imaging synaptic transmission.
This video is DIV12 rat cortical culture with 2uM TTX, imaged with a simple widefield microscope (NA1.4) at 100Hz, displayed here at 20Hz. Flashes correspond to spontaneous release of the neurotransmitter glutamate, monitored using iGluSnFR3.
Kaspar Podgorski (left) and Abhi Aggarwal (right)
Glutamate detection using genetically encoded indicators has come a long way! Here’s a stroll down memory lane showing the development of glutamate biosensors over the past two decades.